Robust expansion of dendritic cells in vivo by hydrodynamic FLT3L-FC gene transfer.
J Immunol Methods
; 413: 69-73, 2014 Nov.
Article
em En
| MEDLINE
| ID: mdl-25066631
ABSTRACT
Due to low numbers of endogenous dendritic cells (DCs) in vivo, exogenous DC-poietin Fms-like tyrosine kinase 3-ligand (FLT3L) is routinely used to generate DC for subsequent studies. We engineered a novel FLT3L-FC DNA construct that, when combined with hydrodynamic gene transfer (HDT), induced robust DC expansion in mice. DC generated in vivo by FLT3L-FC HDT produced cytokines in response to stimulation by an array of TLR agonists and promoted T cell proliferation. The FLT3L-FC protein produced in vivo spontaneously homodimerized to enable effective FLT signaling and the FC-domain enhanced its plasma half-life, providing an improved reagent and method to boost DC numbers.
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Base de dados:
MEDLINE
Assunto principal:
Plasmídeos
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Células Dendríticas
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Proteínas Recombinantes de Fusão
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Técnicas de Transferência de Genes
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Proteínas de Membrana
Limite:
Animals
Idioma:
En
Ano de publicação:
2014
Tipo de documento:
Article