20-OH-ecdysone regulates 60 C beta tubulin gene expression in Kc cells and during Drosophila development.
Exp Cell Res
; 184(1): 241-9, 1989 Sep.
Article
em En
| MEDLINE
| ID: mdl-2507336
ABSTRACT
Cultured Kc cells of Drosophila melanogaster are sensitive to the insect moulting hormone, 20-hydroxy-ecdysone (20-OH-E). Morphological changes of Kc-treated cells were observed and electron microscopic analysis of pseudopodia shows a large increase in the number of microtubules, all arranged in the same orientation. The 60 C beta tubulin gene which is expressed only in 20-OH-E-treated cells encodes a 2.6-kb mRNA which is essentially cytoplasmic and polyadenylated. The corresponding premessenger is 7 kb in length and is absent in untreated cells. Two peaks of expression of the 60 C beta tubulin gene are observed during Drosophila development at midembryogenesis (stage 8-13 h) and at the late third instar larvae-early pupae stage. By use of the Ecdysone 1 mutant, 60 C beta tubulin gene expression was demonstrated to be regulated in part by 20-OH-E during Drosophila development. Through these two complementary biological models of study, the mode and role of beta tubulin gene regulation are discussed.
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Base de dados:
MEDLINE
Assunto principal:
Tubulina (Proteína)
/
Regulação da Expressão Gênica
/
Drosophila melanogaster
/
Ecdisterona
Limite:
Animals
Idioma:
En
Ano de publicação:
1989
Tipo de documento:
Article