delta-Aminolevulinic acid biosynthesis in Escherichia coli and Bacillus subtilis involves formation of glutamyl-tRNA.
FEMS Microbiol Lett
; 51(3): 255-9, 1989 Aug.
Article
em En
| MEDLINE
| ID: mdl-2511063
ABSTRACT
Cell-free extracts of Escherichia coli and Bacillus subtilis catalyzed the tRNA-dependent, RNase A-sensitive formation of delta-aminolevulinic acid (ALA) from glutamate. Cell extracts prepared from cultures of E. coli grown under aerobic or anaerobic conditions had similar levels of ALA biosynthetic activity. Both the tRNA-stimulated conversion of glutamate to ALA and the conversion of glutamate-1-semialdehyde to ALA were inhibited by gabaculin. However, gabaculin had no effect on the growth of either E. coli or B. subtilis. The tRNA-dependent transformation of glutamate to ALA in E. coli and B. subtilis thus appears to be very similar to the pathway found in cyanobacteria, certain obligate anaerobic eubacteria, archaebacteria and in the chloroplasts of algae and higher plant species.
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Base de dados:
MEDLINE
Assunto principal:
Bacillus subtilis
/
RNA de Transferência Aminoácido-Específico
/
RNA de Transferência de Ácido Glutâmico
/
Transferases Intramoleculares
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Escherichia coli
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Ácido Aminolevulínico
/
Ácidos Levulínicos
Idioma:
En
Ano de publicação:
1989
Tipo de documento:
Article