Selective uptake of cylindrical poly(2-oxazoline) brush-antiDEC205 antibody-OVA antigen conjugates into DEC-positive dendritic cells and subsequent T-cell activation.
Chemistry
; 20(39): 12405-10, 2014 Sep 22.
Article
em En
| MEDLINE
| ID: mdl-25111768
ABSTRACT
To achieve specific cell targeting by various receptors for oligosaccharides or antibodies, a carrier must not be taken up by any of the very many different cells and needs functional groups prone to clean conjugation chemistry to derive well-defined structures with a high biological specificity. A polymeric nanocarrier is presented that consists of a cylindrical brush polymer with poly-2-oxazoline side chains carrying an azide functional group on each of the many side chain ends. After click conjugation of dye and an anti-DEC205 antibody to the periphery of the cylindrical brush polymer, antibody-mediated specific binding and uptake into DEC205(+) -positive mouse bone marrow-derived dendritic cells (BMDC) was observed, whereas binding and uptake by DEC205(-) negative BMDC and non-DC was essentially absent. Additional conjugation of an antigen peptide yielded a multifunctional polymer structure with a much stronger antigen-specific T-cell stimulatory capacity of pretreated BMDC than application of antigen or polymer-antigen conjugate.
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Texto completo:
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Base de dados:
MEDLINE
Assunto principal:
Células Dendríticas
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Linfócitos T
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Antígenos CD
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Receptores de Superfície Celular
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Imunoconjugados
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Lectinas Tipo C
Limite:
Animals
Idioma:
En
Ano de publicação:
2014
Tipo de documento:
Article