Brief exposure to cigarette smoke impairs airway epithelial cell innate anti-viral defence.
Toxicol In Vitro
; 28(8): 1430-5, 2014 Dec.
Article
em En
| MEDLINE
| ID: mdl-25111775
ABSTRACT
BACKGROUND:
Human rhinovirus (hRV) infections commonly cause acute upper respiratory infections and asthma exacerbations. Environmental cigarette smoke exposure is associated with a significant increase in the risk for these infections in children.OBJECTIVE:
To determine the impact of short-term exposure to cigarette smoke on innate immune responses of airway epithelial cells infected with hRV.METHODS:
A human bronchial epithelial cell line (HBEC-3KT) was exposed to cigarette smoke extract (CSE) for 30 min and subsequently infected with hRV serotype 1B. Viral-induced cytokine release was measured with AlphaLISA and viral replication quantified by shed viral titer and intracellular viral copy number 24h post-infection.RESULTS:
CSE induced a concentration-dependent decrease in CXCL10 (p<0.001) and IFN-ß (p<0.001), with a 79% reduction at the highest dose with an associated 3-fold increase in shed virus. These effects were maintained when infection was delayed up to 24h post CSE exposure. Exogenous IFN-ß treatment at t=0 after infection blunts the effects of CSE on viral replication (p<0.05).CONCLUSION:
A single exposure of 30 min to cigarette smoke has a lasting impact on epithelial innate defence providing a plausible mechanism for the increase in respiratory infections seen in children exposed to second-hand tobacco smoke.Palavras-chave
Texto completo:
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Base de dados:
MEDLINE
Assunto principal:
Rhinovirus
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Fumaça
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Nicotiana
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Brônquios
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Imunidade Inata
Limite:
Humans
Idioma:
En
Ano de publicação:
2014
Tipo de documento:
Article