Generation and characterization of Lhx9-GFPCreER(T2) knock-in mouse line.
Genesis
; 52(9): 827-832, 2014 Sep.
Article
em En
| MEDLINE
| ID: mdl-25112520
ABSTRACT
LHX9 is a LIM-homeodomain transcription factor essential for the development of gonads, spinal cord interneurons, and thalamic neurons to name a few. We recently reported the expression of LHX9 in retinal amacrine cells during development. In this study, we generated an Lhx9-GFPCreER(T) (2) (GCE) knock-in mouse line by knocking-in a GCE cassette at the Lhx9 locus, thus inactivating endogenous Lhx9. Lhx9(GCE) (/+) mice were viable, fertile, and displayed no overt phenotypical characteristics. Lhx9(GCE) (/) (GCE) mice were all phenotypically female, smaller in size, viable, but infertile. The specificity and efficacy of the Lhx9-GCE mouse line was verified by crossing it to a Rosa26-tdTomato reporter mouse line, which reveals the Cre recombinase activities in retinal amacrine cells, developing limbs, testis, hippocampal neurons, thalamic neurons, and cerebellar neurons. Taken together, the Lhx9-GCE mouse line could serve as a beneficial tool for lineage tracing and gene manipulation experiments. genesis
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Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Fatores de Transcrição
/
Proteínas com Homeodomínio LIM
Limite:
Animals
Idioma:
En
Ano de publicação:
2014
Tipo de documento:
Article