Your browser doesn't support javascript.
loading
Binding of the transcription factor Atf1 to promoters serves as a barrier to phase nucleosome arrays and avoid cryptic transcription.
García, Patricia; Paulo, Esther; Gao, Jun; Wahls, Wayne P; Ayté, José; Lowy, Ernesto; Hidalgo, Elena.
Afiliação
  • García P; Oxidative Stress and Cell Cycle Group, Departament de Ciències Experimentals i de la Salut, Universitat Pompeu Fabra, C/ Dr Aiguader 88, 08003 Barcelona, Spain.
  • Paulo E; Oxidative Stress and Cell Cycle Group, Departament de Ciències Experimentals i de la Salut, Universitat Pompeu Fabra, C/ Dr Aiguader 88, 08003 Barcelona, Spain.
  • Gao J; Department of Biochemistry and Molecular Biology, University of Arkansas for Medical Sciences, 4301 W Markham St., Little Rock, AR 72205, USA.
  • Wahls WP; Department of Biochemistry and Molecular Biology, University of Arkansas for Medical Sciences, 4301 W Markham St., Little Rock, AR 72205, USA.
  • Ayté J; Oxidative Stress and Cell Cycle Group, Departament de Ciències Experimentals i de la Salut, Universitat Pompeu Fabra, C/ Dr Aiguader 88, 08003 Barcelona, Spain.
  • Lowy E; Core Facilities, Centre for Genomic Regulation, Universitat Pompeu Fabra, C/ Dr Aiguader 88, 0800 Barcelona, Spain.
  • Hidalgo E; Oxidative Stress and Cell Cycle Group, Departament de Ciències Experimentals i de la Salut, Universitat Pompeu Fabra, C/ Dr Aiguader 88, 08003 Barcelona, Spain elena.hidalgo@upf.edu.
Nucleic Acids Res ; 42(16): 10351-9, 2014.
Article em En | MEDLINE | ID: mdl-25122751
ABSTRACT
Schizosaccharomyces pombe displays a large transcriptional response common to several stress conditions, regulated primarily by the transcription factor Atf1. Atf1-dependent promoters contain especially broad nucleosome depleted regions (NDRs) prior to stress imposition. We show here that basal binding of Atf1 to these promoters competes with histones to create wider NDRs at stress genes. Moreover, deletion of atf1 results in nucleosome disorganization specifically at stress coding regions and derepresses antisense transcription. Our data indicate that the transcription factor binding to promoters acts as an effective barrier to fix the +1 nucleosome and phase downstream nucleosome arrays to prevent cryptic transcription.
Assuntos

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Fosfoproteínas / Transcrição Gênica / Nucleossomos / Regiões Promotoras Genéticas / Proteínas de Schizosaccharomyces pombe / Fator 1 Ativador da Transcrição Idioma: En Ano de publicação: 2014 Tipo de documento: Article

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Fosfoproteínas / Transcrição Gênica / Nucleossomos / Regiões Promotoras Genéticas / Proteínas de Schizosaccharomyces pombe / Fator 1 Ativador da Transcrição Idioma: En Ano de publicação: 2014 Tipo de documento: Article