RNAi screening comes of age: improved techniques and complementary approaches.
Nat Rev Mol Cell Biol
; 15(9): 591-600, 2014 Sep.
Article
em En
| MEDLINE
| ID: mdl-25145850
ABSTRACT
Gene silencing through sequence-specific targeting of mRNAs by RNAi has enabled genome-wide functional screens in cultured cells and in vivo in model organisms. These screens have resulted in the identification of new cellular pathways and potential drug targets. Considerable progress has been made to improve the quality of RNAi screen data through the development of new experimental and bioinformatics approaches. The recent availability of genome-editing strategies, such as the CRISPR (clustered regularly interspaced short palindromic repeats)-Cas9 system, when combined with RNAi, could lead to further improvements in screen data quality and follow-up experiments, thus promoting our understanding of gene function and gene regulatory networks.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Testes Genéticos
/
RNA Interferente Pequeno
/
Interferência de RNA
/
Redes Reguladoras de Genes
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Sequências Repetidas Invertidas
Tipo de estudo:
Diagnostic_studies
/
Prognostic_studies
/
Screening_studies
Limite:
Animals
/
Humans
Idioma:
En
Ano de publicação:
2014
Tipo de documento:
Article