Transient expression of the angiotensin II receptor: a rapid and functional analysis of a calcium-mobilizing seven-transmembrane domain receptor in COS-7 cells.
Biochem Biophys Res Commun
; 165(3): 935-41, 1989 Dec 29.
Article
em En
| MEDLINE
| ID: mdl-2514688
ABSTRACT
The mas oncogene/angiotensin II receptor was subcloned into a mammalian expression vector pCDM8 and used to transiently transfect monkey kidney derived COS-7 cells. As a result, the mas transfected COS-7 cells expressed a functional angiotensin II receptor capable of transducing an increase in intracellular Ca2+ following stimulation with angiotensin II. The angiotensin II stimulated changes in Ca2+ could be measured 24 hours after transfection in both a fluorimeter and a fluorescence activated cell sorter. These results describe a rapid method for the functional analysis of the 7-transmembrane domain receptor genes.
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Base de dados:
MEDLINE
Assunto principal:
Angiotensina II
/
Receptores de Angiotensina
/
Expressão Gênica
/
Membrana Celular
/
Cálcio
Limite:
Animals
Idioma:
En
Ano de publicação:
1989
Tipo de documento:
Article