Multiplexed programmable release of captured DNA.
Chembiochem
; 15(16): 2353-6, 2014 Nov 03.
Article
em En
| MEDLINE
| ID: mdl-25157426
ABSTRACT
Nucleic-acid hybridization is widely used for the specific capture of complementary sequences from complex samples. It is useful for both analytical methodologies, such as array hybridization (e.g. transcriptome analysis, genetic-variation analysis), and preparative strategies such as exome sequencing and sequence-specific proteome capture and analysis (PICh, HyCCAPP). It has not generally been possible to selectively elute particular captured subsequences, however, as the conditions employed for disruption of a duplex can lack the specificity needed to discriminate between different sequences. We show here that it is possible to bind and selectively release multiple sets of sequences by using toehold-mediated DNA branch migration. The strategy is illustrated for simple mixtures of oligonucleotides, for the sequence-specific capture and specific release of crosslinked yeast chromatin, and for the specific release of oligonucleotides hybridized to DNA microarrays.
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MEDLINE
Assunto principal:
DNA
Idioma:
En
Ano de publicação:
2014
Tipo de documento:
Article