2´,3´-Dialdehyde of ATP, ADP, and adenosine inhibit HIV-1 reverse transcriptase and HIV-1 replication.
Curr HIV Res
; 12(5): 347-58, 2014.
Article
em En
| MEDLINE
| ID: mdl-25174839
ABSTRACT
The 2´3´-dialdehyde of ATP or oxidized ATP (oATP) is a compound known for specifically making covalent bonds with the nucleotide-binding site of several ATP-binding enzymes and receptors. We investigated the effects of oATP and other oxidized purines on HIV-1 infection and we found that this compound inhibits HIV-1 and SIV infection by blocking early steps of virus replication. oATP, oxidized ADP (oADP), and oxidized Adenosine (oADO) impact the natural activity of endogenous reverse transcriptase enzyme (RT) in cell free virus particles and are able to inhibit viral replication in different cell types when added to the cell cultures either before or after infection. We used UFLC-UV to show that both oADO and oATP can be detected in the cell after being added in the extracellular medium. oATP also suppresses RT activity and replication of the HIV-1 resistant variants M184V and T215Y. We conclude that oATP, oADP and oADO display anti HIV-1 activity that is at in least in part due to inhibitory activity on HIV-1 RT.
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Base de dados:
MEDLINE
Assunto principal:
Replicação Viral
/
Infecções por HIV
/
Adenosina
/
Difosfato de Adenosina
/
Trifosfato de Adenosina
/
HIV-1
/
Fármacos Anti-HIV
/
Transcriptase Reversa do HIV
Limite:
Humans
Idioma:
En
Ano de publicação:
2014
Tipo de documento:
Article