[Isolation and identification of cellulolytic anaerobic fungi and their associated methanogens from holstein cow].
Wei Sheng Wu Xue Bao
; 54(5): 563-71, 2014 May 04.
Article
em Zh
| MEDLINE
| ID: mdl-25199255
ABSTRACT
OBJECTIVE:
We studied the microbial interaction between anaerobic fungi and methanogens in the rumen of Holstein Cow.METHODS:
Co-cultures of anaerobic fungi with indigenously associated methanogen were isolated by Hungate roll-tube technique. The anaerobic fungi were identified by morphology and 4', 6 diamidino-2-phylindole nucleus staining and the methanogens were identified by 16S rRNA gene sequencing.RESULTS:
A total of 28 co-cultures of anaerobic fungus with indigenously associated methanogen were obtained. The anaerobic fungi in the co-cultures were identified as monocentric genera Piromyces, Neocallimastix and Caeomyces. The indigenously associated methanogens were Methanobrevibacter olleyae like and Methanobrevibacter thaueri like strains. Four different phylotypes of fungus-methanogen co-cultures were obtained, which were Piromyces/Methanobrevibacter olleyae like strains, Neocallimastix/ Methanobrevibacter olleyae like strains, Neocallimastix/Methanobrevibacter thaueri like strains and Caecomyces/ Methanobrevibacter olleyae like strains.CONCLUSION:
Our study isolated and identified 28 co-cultures of anaerobic fungus and associated methanogens, which provided new materials for further study the mechanism of methane emission in the rumen.
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Base de dados:
MEDLINE
Assunto principal:
Rúmen
/
Bactérias
/
Celulose
/
Fungos
/
Metano
Tipo de estudo:
Diagnostic_studies
/
Risk_factors_studies
Limite:
Animals
Idioma:
Zh
Ano de publicação:
2014
Tipo de documento:
Article