[Screening bioactive compounds inhibiting influenza virus from isatidis radix by ultrafiltration mass spectrometry].
Zhongguo Zhong Yao Za Zhi
; 39(5): 812-6, 2014 Mar.
Article
em Zh
| MEDLINE
| ID: mdl-25204170
ABSTRACT
In vitro neuraminidase inhibition assays and ultrafiltration liquid chromatography with diodearray detector coupled to time of flight mass spectrometer (UPLC-DAD-TOF-MS) were combined to screen bioactive compounds inhibiting neuraminidase from Isatidis Radix. By comparing the compounds from Isatidis Radix before and after ultrafiltration, we found that arginine, goitrin and adenosinea can bind with neuraminidase, and the binding degree of the three compounds were (36.23 +/- 1.12)%, (32.54 +/- 1.02)% and (9.38 +/- 0.47)%, respectively. The IC50 of arginine and goitrin were (1.16 +/- 0.02), (1.20 +/- 0.02) g x L(-1), respectively. While the IC50 of adenosinea was higher than 500 g x L(-1). The results showed that arginine and goitrin might be the main compounds with antiviral activity of Isatidis Radix. This study may provide a useful method for the screening of bioactive compounds and quality control of Isatidis Radix.
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Base de dados:
MEDLINE
Assunto principal:
Antivirais
/
Orthomyxoviridae
/
Medicamentos de Ervas Chinesas
/
Raízes de Plantas
/
Isatis
Tipo de estudo:
Diagnostic_studies
/
Screening_studies
Idioma:
Zh
Ano de publicação:
2014
Tipo de documento:
Article