Lineage-specific expression of a T cell receptor variable gene promoter controlled by upstream sequences.
J Exp Med
; 169(4): 1213-31, 1989 Apr 01.
Article
em En
| MEDLINE
| ID: mdl-2522500
ABSTRACT
We have characterized the sequence contribution of DNA 5' of a functionally rearranged TCR promoter (V beta 8.1) on its T lineage-specific expression through the use of the chloramphenicol acetyl-transferase (CAT) reporter gene. A 230-bp fragment located 570 bp upstream of the determined transcription start site of the V beta 8.1 promoter confers a T lineage specificity of expression to a heterologous promoter. The inability of the V beta 8.1 promoter and its associated elements to function in B cells suggests the existence of a mechanism to prevent inappropriate V beta gene expression in B cells. Of considerable interest is the fact that both a B cell-specific and a nontissue-specific enhancer element were incapable of stimulating significant expression of this promoter in B cells. We discuss the implication of these results on the process of rearrangement of both Ig and TCR genes, and the differentiation of the lymphoid system.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Receptores de Antígenos de Linfócitos T
/
Linfócitos T
/
Regulação da Expressão Gênica
/
Regiões Promotoras Genéticas
Limite:
Humans
Idioma:
En
Ano de publicação:
1989
Tipo de documento:
Article