Function and dynamics of macromolecular complexes explored by integrative structural and computational biology.
Curr Opin Struct Biol
; 27: 138-48, 2014 Aug.
Article
em En
| MEDLINE
| ID: mdl-25238653
ABSTRACT
Three vignettes exemplify the potential of combining EM and X-ray crystallographic data with molecular dynamics (MD) simulation to explore the architecture, dynamics and functional properties of multicomponent, macromolecular complexes. The first two describe how EM and X-ray crystallography were used to solve structures of the ribosome and the Arp2/3-actin complex, which enabled MD simulations that elucidated functional dynamics. The third describes how EM, X-ray crystallography, and microsecond MD simulations of a GPCRG protein complex were used to explore transmembrane signaling by the ß-adrenergic receptor. Recent technical advancements in EM, X-ray crystallography and computational simulation create unprecedented synergies for integrative structural biology to reveal new insights into heretofore intractable biological systems.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Biologia Computacional
/
Substâncias Macromoleculares
Limite:
Animals
/
Humans
Idioma:
En
Ano de publicação:
2014
Tipo de documento:
Article