Cdc42 and formin activity control non-muscle myosin dynamics during Drosophila heart morphogenesis.
J Cell Biol
; 206(7): 909-22, 2014 Sep 29.
Article
em En
| MEDLINE
| ID: mdl-25267295
ABSTRACT
During heart formation, a network of transcription factors and signaling pathways guide cardiac cell fate and differentiation, but the genetic mechanisms orchestrating heart assembly and lumen formation remain unclear. Here, we show that the small GTPase Cdc42 is essential for Drosophila melanogaster heart morphogenesis and lumen formation. Cdc42 genetically interacts with the cardiogenic transcription factor tinman; with dDAAM which belongs to the family of actin organizing formins; and with zipper, which encodes nonmuscle myosin II. Zipper is required for heart lumen formation, and its spatiotemporal activity at the prospective luminal surface is controlled by Cdc42. Heart-specific expression of activated Cdc42, or the regulatory formins dDAAM and Diaphanous caused mislocalization of Zipper and induced ectopic heart lumina, as characterized by luminal markers such as the extracellular matrix protein Slit. Placement of Slit at the lumen surface depends on Cdc42 and formin function. Thus, Cdc42 and formins play pivotal roles in heart lumen formation through the spatiotemporal regulation of the actomyosin network.
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Base de dados:
MEDLINE
Assunto principal:
Proteínas de Transporte
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Proteínas de Ligação ao GTP
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Miosina Tipo II
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Proteínas de Drosophila
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Proteínas Adaptadoras de Transdução de Sinal
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Drosophila melanogaster
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Coração
Limite:
Animals
Idioma:
En
Ano de publicação:
2014
Tipo de documento:
Article