Phenylalanine binding is linked to dimerization of the regulatory domain of phenylalanine hydroxylase.
Biochemistry
; 53(42): 6625-7, 2014 Oct 28.
Article
em En
| MEDLINE
| ID: mdl-25299136
ABSTRACT
Analytical ultracentrifugation has been used to analyze the oligomeric structure of the isolated regulatory domain of phenylalanine hydroxylase. The protein exhibits a monomer-dimer equilibrium with a dissociation constant of ~46 µM; this value is unaffected by the removal of the 24 N-terminal residues or by phosphorylation of Ser16. In contrast, phenylalanine binding (Kd = 8 µM) stabilizes the dimer. These results suggest that dimerization of the regulatory domain of phenylalanine hydroxylase is linked to allosteric activation of the enzyme.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Fenilalanina Hidroxilase
/
Fenilalanina
Idioma:
En
Ano de publicação:
2014
Tipo de documento:
Article