Rapid modification of the pET-28 expression vector for ligation independent cloning using homologous recombination in Saccharomyces cerevisiae.

Gay, Glen; Wagner, Drew T; Keatinge-Clay, Adrian T; Gay, Darren C

Gay, Glen; Wagner, Drew T; Keatinge-Clay, Adrian T; Gay, Darren C.

Afiliação

Gay G; Department of Molecular Biosciences, University of Texas at Austin, Austin, TX 78712, United States.
Wagner DT; Department of Molecular Biosciences, University of Texas at Austin, Austin, TX 78712, United States.
Keatinge-Clay AT; Department of Molecular Biosciences, University of Texas at Austin, Austin, TX 78712, United States.
Gay DC; Department of Molecular Biosciences, University of Texas at Austin, Austin, TX 78712, United States. Electronic address: darren.gay@utexas.edu.

Plasmid ; 76: 66-71, 2014 11.

Article em En | MEDLINE | ID: mdl-25304917

Assuntos

Clonagem Molecular/métodos; Vetores Genéticos/genética; Recombinação Homóloga; Saccharomyces cerevisiae/genética; Primers do DNA; Reação em Cadeia da Polimerase/métodos

Palavras-chave

Homologous recombination; Ligation-independent cloning; Plasmid assembly; Plasmid reconstruction

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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Saccharomyces cerevisiae / Clonagem Molecular / Recombinação Homóloga / Vetores Genéticos Idioma: En Ano de publicação: 2014 Tipo de documento: Article

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