A new ester of fatty acid from a methanol extract of the whole plant of Amaranthus spinosus and its α-glucosidase inhibitory activity.
Pharm Biol
; 53(4): 600-4, 2015 Apr.
Article
em En
| MEDLINE
| ID: mdl-25339411
ABSTRACT
CONTEXT Amaranthus spinosus Linn. (Amaranthaceae), commonly known as "spiny pigweed", is used both in the Indian traditional system and in folk medicine to treat diabetes. OBJECTIVE:
The present study evaluates the scientific basis of antidiabetic activity of chloroform fraction of methanol extract of A. spinosus and of an isolated constituent of A. spinosus. MATERIALS ANDMETHODS:
HPLC analysis was performed to determine the purity and the amount of the constituent present in the plant extract. The yeast α-glucosidase inhibition technique was used to determine the antidiabetic activity of A. spinosus. Acarbose was used as a standard. An appropriate therapeutic approach for preventing diabetes mellitus and obesity is to retard the absorption of glucose by inhibition of α-glucosidase.RESULTS:
One novel fatty acid with strong α-glucosidase inhibitory activity - (14E, 18E, 22E, 26E) - methyl nonacosa-14, 18, 22, 26 tetraenoate [1] (IC50 value 6.52 µM/mL) and ß-sitosterol [2] were purified. Compound 1 was found to be more potent than the methanol extract. HPLC quantitative analysis revealed that 0.15% of compound 1 and 0.06% of compound 2 were present in the plant extract.CONCLUSION:
This novel fatty acid can potentially be developed as a novel natural nutraceutical for the management of diabetes.Palavras-chave
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Base de dados:
MEDLINE
Assunto principal:
Sitosteroides
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Extratos Vegetais
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Amaranthus
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Metanol
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Ácidos Graxos Insaturados
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Inibidores de Glicosídeo Hidrolases
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Hipoglicemiantes
Idioma:
En
Ano de publicação:
2015
Tipo de documento:
Article