Microsatellite abundance across the Anthozoa and Hydrozoa in the phylum Cnidaria.
BMC Genomics
; 15: 939, 2014 Oct 27.
Article
em En
| MEDLINE
| ID: mdl-25346285
ABSTRACT
BACKGROUND:
Microsatellite loci have high mutation rates and thus are indicative of mutational processes within the genome. By concentrating on the symbiotic and aposymbiotic cnidarians, we investigated if microsatellite abundances follow a phylogenetic or ecological pattern. Individuals from eight species were shotgun sequenced using 454 GS-FLX Titanium technology. Sequences from the three available cnidarian genomes (Nematostella vectensis, Hydra magnipapillata and Acropora digitifera) were added to the analysis for a total of eleven species representing two classes, three subclasses and eight orders within the phylum Cnidaria.RESULTS:
Trinucleotide and tetranucleotide repeats were the most abundant motifs, followed by hexa- and dinucleotides. Pentanucleotides were the least abundant motif in the data set. Hierarchical clustering and log likelihood ratio tests revealed a weak relationship between phylogeny and microsatellite content. Further, comparisons between cnidaria harboring intracellular dinoflagellates and those that do not, show microsatellite coverage is higher in the latter group.CONCLUSIONS:
Our results support previous studies that found tri- and tetranucleotides to be the most abundant motifs in invertebrates. Differences in microsatellite coverage and composition between symbiotic and non-symbiotic cnidaria suggest the presence/absence of dinoflagellates might place restrictions on the host genome.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Repetições de Microssatélites
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Antozoários
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Hidrozoários
Limite:
Animals
Idioma:
En
Ano de publicação:
2014
Tipo de documento:
Article