Building carbon-carbon bonds using a biocatalytic methanol condensation cycle.
Proc Natl Acad Sci U S A
; 111(45): 15928-33, 2014 Nov 11.
Article
em En
| MEDLINE
| ID: mdl-25355907
ABSTRACT
Methanol is an important intermediate in the utilization of natural gas for synthesizing other feedstock chemicals. Typically, chemical approaches for building C-C bonds from methanol require high temperature and pressure. Biological conversion of methanol to longer carbon chain compounds is feasible; however, the natural biological pathways for methanol utilization involve carbon dioxide loss or ATP expenditure. Here we demonstrated a biocatalytic pathway, termed the methanol condensation cycle (MCC), by combining the nonoxidative glycolysis with the ribulose monophosphate pathway to convert methanol to higher-chain alcohols or other acetyl-CoA derivatives using enzymatic reactions in a carbon-conserved and ATP-independent system. We investigated the robustness of MCC and identified operational regions. We confirmed that the pathway forms a catalytic cycle through (13)C-carbon labeling. With a cell-free system, we demonstrated the conversion of methanol to ethanol or n-butanol. The high carbon efficiency and low operating temperature are attractive for transforming natural gas-derived methanol to longer-chain liquid fuels and other chemical derivatives.
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Base de dados:
MEDLINE
Assunto principal:
Dióxido de Carbono
/
Trifosfato de Adenosina
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Metanol
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Modelos Químicos
Idioma:
En
Ano de publicação:
2014
Tipo de documento:
Article