[Physical characterization of decellularized cartilage matrix for reconstructive rhinosurgery]. / Physikalische Charakterisierung dezellularisierter Knorpelmatrix zur Anwendung in der Rhinochirurgie.
Laryngorhinootologie
; 93(11): 756-63, 2014 Nov.
Article
em De
| MEDLINE
| ID: mdl-25369160
ABSTRACT
BACKGROUND:
The use of autologous auricular and rib cartilage for the reconstruction of nasal defects and deformities is associated with a number of disadvantages. The development of alternative materials is therefore the focus of intensive research. Recent studies demonstrated that decellularized cartilage is a promising material for cartilage tissue engineering. Hence, the aim of this study was to characterize the materials surface and cellular reactions to the decellularized cartilage matrix in long term-3D-culture. MATERIAL ANDMETHODS:
Material geometry of decellularized cartilage was examined by microcomputed tomography as well as material characteristics by scanning and transmission electron microscopy. The expression of integrins on the surface of human chondrocytes was determined after seeding and migration into the scaffold.RESULTS:
After decellularization an obvious enlargement of the matrix surface and an intensive interaction between the chondrocytes and the collagen matrix was observed. ITGA1 and ITGB1 were upregulated indicating chondrogenic differentiation.CONCLUSION:
Therefore, decellularized porcine cartilage provides an optimal microstructure for human chondrocytes with respect to cell integration and matrix production. Thus, it offers promising characteristics for clinical application in reconstructive surgery.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Rinoplastia
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Cartilagem
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Microscopia Eletrônica de Transmissão e Varredura
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Microtomografia por Raio-X
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Proteínas Matrilinas
Limite:
Humans
Idioma:
De
Ano de publicação:
2014
Tipo de documento:
Article