Towards imaging metabolic pathways in tissues.
Anal Bioanal Chem
; 407(8): 2167-76, 2015 Mar.
Article
em En
| MEDLINE
| ID: mdl-25381617
ABSTRACT
Matrix-assisted laser desorption/ionization (MALDI) mass spectrometry imaging using 9-aminoacridine as the matrix leads to the detection of low mass metabolites and lipids directly from cancer tissues. These included lactate and pyruvate for studying the Warburg effect, as well as succinate and fumarate, metabolites whose accumulation is associated with specific syndromes. By using the pathway information present in the human metabolome database, it was possible to identify regions within tumor tissue samples with distinct metabolic signatures that were consistent with known tumor biology. We present a data analysis workflow for assessing metabolic pathways in their histopathological context.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Neoplasias da Mama
/
Neoplasias da Glândula Tireoide
/
Espectrometria de Massas por Ionização e Dessorção a Laser Assistida por Matriz
/
Redes e Vias Metabólicas
Tipo de estudo:
Evaluation_studies
Limite:
Female
/
Humans
/
Male
Idioma:
En
Ano de publicação:
2015
Tipo de documento:
Article