Uniform and scalable light-sheets generated by extended focusing.
Opt Express
; 22(21): 26141-52, 2014 Oct 20.
Article
em En
| MEDLINE
| ID: mdl-25401646
ABSTRACT
Light-sheet fluorescence microscopy (LSFM) affords highly parallelized 3D imaging with optical sectioning capability and minimal light exposure. However, using Gaussian beams for light-sheet generation results in a trade-off between beam waist thickness and the area over which the beam can approximate a light-sheet. Here, we present a novel form of LSFM that uses incoherent extended focusing to produce divergence free light-sheets with near diffraction-limited resolution and uniform intensity distribution along the propagation direction. We demonstrate the imaging performance of the new technique by volumetric imaging of beads, collagen fibers, and melanoma cancer cells with sub-cellular resolution.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Iluminação
/
Imageamento Tridimensional
/
Luz
/
Microscopia de Fluorescência
Idioma:
En
Ano de publicação:
2014
Tipo de documento:
Article