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LysargiNase mirrors trypsin for protein C-terminal and methylation-site identification.
Huesgen, Pitter F; Lange, Philipp F; Rogers, Lindsay D; Solis, Nestor; Eckhard, Ulrich; Kleifeld, Oded; Goulas, Theodoros; Gomis-Rüth, F Xavier; Overall, Christopher M.
Afiliação
  • Huesgen PF; 1] Centre for Blood Research, University of British Columbia, Vancouver, British Columbia, Canada. [2] Department of Oral Biological and Medical Sciences, University of British Columbia, Vancouver, British Columbia, Canada. [3] Department of Biochemistry and Molecular Biology, University of British
  • Lange PF; 1] Centre for Blood Research, University of British Columbia, Vancouver, British Columbia, Canada. [2] Department of Oral Biological and Medical Sciences, University of British Columbia, Vancouver, British Columbia, Canada. [3] Department of Biochemistry and Molecular Biology, University of British
  • Rogers LD; 1] Centre for Blood Research, University of British Columbia, Vancouver, British Columbia, Canada. [2] Department of Oral Biological and Medical Sciences, University of British Columbia, Vancouver, British Columbia, Canada. [3] Department of Biochemistry and Molecular Biology, University of British
  • Solis N; 1] Centre for Blood Research, University of British Columbia, Vancouver, British Columbia, Canada. [2] Department of Oral Biological and Medical Sciences, University of British Columbia, Vancouver, British Columbia, Canada. [3] Department of Biochemistry and Molecular Biology, University of British
  • Eckhard U; 1] Centre for Blood Research, University of British Columbia, Vancouver, British Columbia, Canada. [2] Department of Oral Biological and Medical Sciences, University of British Columbia, Vancouver, British Columbia, Canada. [3] Department of Biochemistry and Molecular Biology, University of British
  • Kleifeld O; Department of Biochemistry and Molecular Biology, Monash University, Clayton, Victoria, Australia.
  • Goulas T; Proteolysis Laboratory, Department of Structural Biology, Molecular Biology Institute of Barcelona, Spanish Research Council, Barcelona, Spain.
  • Gomis-Rüth FX; Proteolysis Laboratory, Department of Structural Biology, Molecular Biology Institute of Barcelona, Spanish Research Council, Barcelona, Spain.
  • Overall CM; 1] Centre for Blood Research, University of British Columbia, Vancouver, British Columbia, Canada. [2] Department of Oral Biological and Medical Sciences, University of British Columbia, Vancouver, British Columbia, Canada. [3] Department of Biochemistry and Molecular Biology, University of British
Nat Methods ; 12(1): 55-8, 2015 Jan.
Article em En | MEDLINE | ID: mdl-25419962
ABSTRACT
To improve proteome coverage and protein C-terminal identification, we characterized the Methanosarcina acetivorans thermophilic proteinase LysargiNase, which cleaves before lysine and arginine up to 55 °C. Unlike trypsin, LysargiNase-generated peptides had N-terminal lysine or arginine residues and fragmented with b ion-dominated spectra. This improved protein C terminal-peptide identification and several arginine-rich phosphosite assignments. Notably, cleavage also occurred at methylated or dimethylated lysine and arginine, facilitating detection of these epigenetic modifications.
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Proteômica / Metaloproteases Tipo de estudo: Diagnostic_studies Idioma: En Ano de publicação: 2015 Tipo de documento: Article
Texto completo
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Proteômica / Metaloproteases Tipo de estudo: Diagnostic_studies Idioma: En Ano de publicação: 2015 Tipo de documento: Article