Andrographolide activates the canonical Wnt signalling pathway by a mechanism that implicates the non-ATP competitive inhibition of GSK-3ß: autoregulation of GSK-3ß in vivo.
Biochem J
; 466(2): 415-30, 2015 Mar 01.
Article
em En
| MEDLINE
| ID: mdl-25423492
ABSTRACT
Wnt/ß-catenin signalling is an important pathway that regulates multiple biological processes, including cell adhesion and determination of cell fate during animal development; in the adult nervous system it regulates the structure and function of synapses. Wnt-signalling dysfunction is associated with several neurodegenerative diseases such as schizophrenia and Alzheimer's disease. The use of natural compounds is an interesting strategy in the search for drugs with the therapeutic potential to activate this signalling pathway. In the present study, we report that andrographolide (ANDRO), a component of Andrographis paniculata, is a potent activator of Wnt signalling. Our results indicate that ANDRO activates this pathway, inducing the transcription of Wnt target genes by a mechanism that bypasses Wnt ligand binding to its receptor. In vitro kinase assays demonstrate that ANDRO inhibits glycogen synthase kinase (GSK)-3ß by a non-ATP-competitive, substrate-competitive mode of action. In silico analyses suggest that ANDRO interacts with the substrate-binding site of GSK-3ß. Finally, we demonstrated that the increase seen in the levels of GSK-3ß phosphorylated at Ser9 is the result of an autoregulatory mechanism of the kinase in vivo, although not through activation of protein phosphatase type 1. Our results suggest that ANDRO could be used as a potential therapeutic drug for disorders caused by Wnt-signalling dysfunction such as neurodegenerative diseases.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Nootrópicos
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Quinase 3 da Glicogênio Sintase
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Inibidores de Proteínas Quinases
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Diterpenos
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Via de Sinalização Wnt
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Hipocampo
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Neurônios
Limite:
Animals
Idioma:
En
Ano de publicação:
2015
Tipo de documento:
Article