Sensitive electrochemical biosensing of H2O2 based on cobalt nanoparticles synthesised in iron storage protein molecules, ferritin.
IET Nanobiotechnol
; 8(4): 196-200, 2014 Dec.
Article
em En
| MEDLINE
| ID: mdl-25429497
ABSTRACT
In this report, a highly sensitive electrochemical biosensor based on cobaltferritin immobilised on a self-assembled monolayer modiï¬ed gold electrode for determination of hydrogen peroxide (H2O2) in phosphate buffer solution (pH 7.5) was investigated. The modiï¬ed electrode showed excellent electrochemical activity for oxidation of H2O2. The response to H2O2 on the modiï¬ed electrode was examined using linear sweep and differential pulse voltammetries. In phosphate buffer (pH 7.5, 0.1 M), the fabricated biosensor exhibited a linear dependence (R = 0.989) on the concentration of H2O2 from 2.49 × 10(-9) to 1.91 × 10(-8) M, a high sensitivity of -0.4099 µA/nM and detection limit of 2.48 × 10(-9) based on a signal-to-noise ratio of 3. Charge transfer coefï¬cient (α) and the exchange current (i0) of oxidation for H2O2 were found to be 0.57 and 7.55 A, respectively. It has been shown that, this modiï¬ed electrode is able to determine H2O2 with a high sensitivity, low detection limit and high selectivity.
Texto completo:
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Base de dados:
MEDLINE
Assunto principal:
Técnicas Biossensoriais
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Cobalto
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Nanopartículas Metálicas
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Ferritinas
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Peróxido de Hidrogênio
Tipo de estudo:
Diagnostic_studies
Idioma:
En
Ano de publicação:
2014
Tipo de documento:
Article