Capillary-channeled polymer (C-CP) fibers for the rapid extraction of proteins from urine matrices prior to detection with MALDI-MS.
Proteomics Clin Appl
; 9(5-6): 522-30, 2015 Jun.
Article
em En
| MEDLINE
| ID: mdl-25450308
ABSTRACT
PURPOSE:
While MS is a powerful tool for biomarker determinations, the high salt content and the small molecules present in urine poses incredible challenges. Separation/extraction methods must be employed for the isolation of target species at relevant concentrations. Micropipette tips packed with capillary-channeled polymer (C-CP) fibers are employed for the SPE of proteins from a synthetic and a certified urine matrix. EXPERIMENTALDESIGN:
Extractions are performed utilizing a very simple centrifugation method to spin-down species through the C-CP fiber tips. Proteins adsorb to the hydrophobic polypropylene fibers and are eluted in a solvent suitable for MALDI-MS analysis. Figures of merit are determined for representative compounds ß2-microglobulin, retinol binding protein, and transferrin.RESULTS:
The optimum protein processing included a 100 µL aqueous rinse and an elution solvent composition was 10 µL of 5545 ACNwater (with triflouroacetic acid). MALDI-MS responses for the target proteins are improved from nondetectable levels to eventually yield LOD ranging from 5 to 180 nM in 1 µL aliquots. CONCLUSION AND CLINICAL RELEVANCE C-CP fiber tips offer a plethora of advantages including low materials costs, high throughput, microvolume processing, and the determination of sub-nanogram quantities of analyte; allowing determination of biomarkers that are otherwise undetectable in urine matrices.Palavras-chave
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Proteinúria
/
Urinálise
/
Proteoma
Tipo de estudo:
Diagnostic_studies
Limite:
Humans
Idioma:
En
Ano de publicação:
2015
Tipo de documento:
Article