A hybrid synthetic pathway for butanol production by a hyperthermophilic microbe.
Metab Eng
; 27: 101-106, 2015 Jan.
Article
em En
| MEDLINE
| ID: mdl-25461832
ABSTRACT
Biologically produced alcohols are of great current interest for renewable solvents and liquid transportation fuels. While bioethanol is now produced on a massive scale, butanol has superior fuel characteristics and an additional value as a solvent and chemical feedstock. Butanol production has been demonstrated at ambient temperatures in metabolically-engineered mesophilic organisms, but the ability to engineer a microbe for in vivo high-temperature production of commodity chemicals has several distinct advantages. These include reduced contamination risk, facilitated removal of volatile products, and a wide temperature range to modulate and balance both the engineered pathway and the host׳s metabolism. We describe a synthetic metabolic pathway assembled from genes obtained from three different sources for conversion of acetyl-CoA to 1-butanol, and 1-butanol generation from glucose was demonstrated near 70°C in a microorganism that grows optimally near 100°C. The module could also be used in thermophiles capable of degrading plant biomass.
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MEDLINE
Assunto principal:
1-Butanol
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Thermoanaerobacterium
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Engenharia Metabólica
Idioma:
En
Ano de publicação:
2015
Tipo de documento:
Article