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Perdeuteration: improved visualization of solvent structure in neutron macromolecular crystallography.
Fisher, S J; Blakeley, M P; Howard, E I; Petit-Haertlein, I; Haertlein, M; Mitschler, A; Cousido-Siah, A; Salvay, A G; Popov, A; Muller-Dieckmann, C; Petrova, T; Podjarny, A.
Afiliação
  • Fisher SJ; Institut Laue-Langevin, 71 Avenue des Martyrs, 38000 Grenoble, France.
  • Blakeley MP; Institut Laue-Langevin, 71 Avenue des Martyrs, 38000 Grenoble, France.
  • Howard EI; IFLYSIB, UNLP-CONICET, Calle 59, 789, B1900BTE La Plata, Argentina.
  • Petit-Haertlein I; Institut Laue-Langevin, 71 Avenue des Martyrs, 38000 Grenoble, France.
  • Haertlein M; Institut Laue-Langevin, 71 Avenue des Martyrs, 38000 Grenoble, France.
  • Mitschler A; Department of Integrative Biology, IGBMC, CNRS, INSERM, Université de Strasbourg, 1 Rue Laurent Fries, Illkirch, France.
  • Cousido-Siah A; Department of Integrative Biology, IGBMC, CNRS, INSERM, Université de Strasbourg, 1 Rue Laurent Fries, Illkirch, France.
  • Salvay AG; IFLYSIB, UNLP-CONICET, Calle 59, 789, B1900BTE La Plata, Argentina.
  • Popov A; ESRF, 6 Rue Jules Horowitz, 38043 Grenoble, France.
  • Muller-Dieckmann C; ESRF, 6 Rue Jules Horowitz, 38043 Grenoble, France.
  • Petrova T; Institute of Mathematical Problems of Biology, Russian Academy of Sciences, Pushchino 142290, Russian Federation.
  • Podjarny A; Department of Integrative Biology, IGBMC, CNRS, INSERM, Université de Strasbourg, 1 Rue Laurent Fries, Illkirch, France.
Acta Crystallogr D Biol Crystallogr ; 70(Pt 12): 3266-72, 2014 Dec 01.
Article em En | MEDLINE | ID: mdl-25478844
ABSTRACT
The 1.8 Šresolution neutron structure of deuterated type III antifreeze protein in which the methyl groups of leucine and valine residues are selectively protonated is presented. Comparison between this and the 1.85 Šresolution neutron structure of perdeuterated type III antifreeze protein indicates that perdeuteration improves the visibility of solvent molecules located in close vicinity to hydrophobic residues, as cancellation effects between H atoms of the methyl groups and nearby heavy-water molecules (D2O) are avoided.
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Perciformes / Proteínas Anticongelantes Tipo III / Proteínas de Peixes / Difração de Nêutrons Limite: Animals Idioma: En Ano de publicação: 2014 Tipo de documento: Article
Texto completo
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Perciformes / Proteínas Anticongelantes Tipo III / Proteínas de Peixes / Difração de Nêutrons Limite: Animals Idioma: En Ano de publicação: 2014 Tipo de documento: Article