Intramolecular complementation of measles virus fusion protein stability confers cell-cell fusion activity at 37 °C.
FEBS Lett
; 589(1): 152-8, 2015 Jan 02.
Article
em En
| MEDLINE
| ID: mdl-25479085
ABSTRACT
The fusion (F) protein of measles virus mediates membrane fusion. In this study, we investigated the molecular basis of the cell-cell fusion activity of the F protein. The N465H substitution in the heptad repeat B domain of the stalk region of the F protein eliminates this activity, but an additional mutation in the DIII domain of the head region - N183D, F217L, P219S, I225T or G240R - restores cell-cell fusion. Thermodynamically stabilized by the N465H substitution, the F protein required elevated temperature as high as 40 °C to promote cell-cell fusion, whereas all five DIII mutations caused destabilization of the F protein allowing the highest fusion activity at 30 °C. Stability complementation between the two domains conferred an efficient cell-cell fusion activity on the F protein at 37 °C.
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Base de dados:
MEDLINE
Assunto principal:
Proteínas Virais de Fusão
/
Mutação de Sentido Incorreto
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Vírus do Sarampo
Limite:
Animals
Idioma:
En
Ano de publicação:
2015
Tipo de documento:
Article