MicroRNA-145 inhibits lung cancer cell metastasis.
Mol Med Rep
; 11(4): 3108-14, 2015 Apr.
Article
em En
| MEDLINE
| ID: mdl-25483817
ABSTRACT
Previous studies have identified a variety of microRNAs (miRNAs) that have important roles in cancer progression, particularly in tumor invasion and metastasis. Downregulation of miR145 was reported to occur in various types of human cancer; however, the role of miR145 in lung cancer metastasis and its potential mechanisms of action remain to be elucidated. The present study aimed to investigate the effects of miR145 on metastasis and epithelialmesenchymal transition (EMT) in A549 human lung adenocarcinoma cells. In addition, the underlying mechanisms by which miR145 regulates EMT were examined. The miR145 mimic was transfected into A549 cells; cell invasion and adhesion assays were then performed in order to investigate cell metastasis, and western blot analysis was used to examine the expression of EMT markers. In order to further examine the underlying mechanisms by which miR145 regulates EMT, a luciferase reporter assay was performed to determine whether miR145 targeted Oct4. In addition, the expression of Wnt3a and ßcatenin in A549 cells was measured following transfection with small hairpin RNAOct4. To the best of our knowledge, the results of the present study demonstrated for the first time, that miR145 inhibited lung cancer cell metastasis and EMT via targeting the Oct4 mediated Wnt/ßcatenin signaling pathway.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
MicroRNAs
/
Neoplasias Pulmonares
Tipo de estudo:
Prognostic_studies
Limite:
Humans
Idioma:
En
Ano de publicação:
2015
Tipo de documento:
Article