Synthesis of magnetite/graphene oxide/chitosan composite and its application for protein adsorption.
Mater Sci Eng C Mater Biol Appl
; 45: 8-14, 2014 Dec.
Article
em En
| MEDLINE
| ID: mdl-25491795
ABSTRACT
In this study, a facile and novel strategy was developed to fabricate magnetite/graphene oxide/chitosan (Fe3O4/GO/CS) composite, and the composite was used as a magnetic adsorbent for the enrichment of protein, and followed by matrix-assisted laser desorption ionization mass spectrometry (MALDI-TOF MS) analysis. The phase composition, chemical structure and morphology of the composite were characterized by X-ray diffraction (XRD), Fourier transform infrared spectrometer (FTIR), transmission electron microscopy (TEM), scanning electronic microscope (SEM) and vibrating sample magnetometer (VSM). Protein cytochrome c was chosen as model target to evaluate the adsorptive property of Fe3O4/GO/CS. After enrichment procedure and magnetic separation, protein bounded with the material was analyzed by MALDI-TOF MS without desorption. The results indicated that Fe3O4/GO/CS composite exhibited a good adsorptive capacity for protein, and Fe3O4/GO/CS composite had a promising potential in magnetic separation research.
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Texto completo:
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Base de dados:
MEDLINE
Assunto principal:
Citocromos c
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Quitosana
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Nanoestruturas
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Óxido Ferroso-Férrico
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Grafite
Idioma:
En
Ano de publicação:
2014
Tipo de documento:
Article