Your browser doesn't support javascript.
loading
Non-coding roX RNAs prevent the binding of the MSL-complex to heterochromatic regions.
Figueiredo, Margarida L A; Kim, Maria; Philip, Philge; Allgardsson, Anders; Stenberg, Per; Larsson, Jan.
Afiliação
  • Figueiredo ML; Department of Molecular Biology, Umeå University, Umeå, Sweden.
  • Kim M; Department of Molecular Biology, Umeå University, Umeå, Sweden.
  • Philip P; Department of Molecular Biology, Umeå University, Umeå, Sweden; Computational Life Science Cluster (CLiC), Umeå University, Umeå, Sweden.
  • Allgardsson A; Department of Molecular Biology, Umeå University, Umeå, Sweden.
  • Stenberg P; Department of Molecular Biology, Umeå University, Umeå, Sweden; Computational Life Science Cluster (CLiC), Umeå University, Umeå, Sweden.
  • Larsson J; Department of Molecular Biology, Umeå University, Umeå, Sweden.
PLoS Genet ; 10(12): e1004865, 2014 Dec.
Article em En | MEDLINE | ID: mdl-25501352
Long non-coding RNAs contribute to dosage compensation in both mammals and Drosophila by inducing changes in the chromatin structure of the X-chromosome. In Drosophila melanogaster, roX1 and roX2 are long non-coding RNAs that together with proteins form the male-specific lethal (MSL) complex, which coats the entire male X-chromosome and mediates dosage compensation by increasing its transcriptional output. Studies on polytene chromosomes have demonstrated that when both roX1 and roX2 are absent, the MSL-complex becomes less abundant on the male X-chromosome and is relocated to the chromocenter and the 4th chromosome. Here we address the role of roX RNAs in MSL-complex targeting and the evolution of dosage compensation in Drosophila. We performed ChIP-seq experiments which showed that MSL-complex recruitment to high affinity sites (HAS) on the X-chromosome is independent of roX and that the HAS sequence motif is conserved in D. simulans. Additionally, a complete and enzymatically active MSL-complex is recruited to six specific genes on the 4th chromosome. Interestingly, our sequence analysis showed that in the absence of roX RNAs, the MSL-complex has an affinity for regions enriched in Hoppel transposable elements and repeats in general. We hypothesize that roX mutants reveal the ancient targeting of the MSL-complex and propose that the role of roX RNAs is to prevent the binding of the MSL-complex to heterochromatin.
Assuntos

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Fatores de Transcrição / Proteínas Nucleares / Heterocromatina / Proteínas de Drosophila / Proteínas de Ligação a DNA / Drosophila melanogaster Limite: Animals Idioma: En Ano de publicação: 2014 Tipo de documento: Article

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Fatores de Transcrição / Proteínas Nucleares / Heterocromatina / Proteínas de Drosophila / Proteínas de Ligação a DNA / Drosophila melanogaster Limite: Animals Idioma: En Ano de publicação: 2014 Tipo de documento: Article