Chromatographic and spectroscopic analysis of the components present in the phenanthridinium trypanocidal agent isometamidium.
Anal Bioanal Chem
; 407(4): 1171-80, 2015 Feb.
Article
em En
| MEDLINE
| ID: mdl-25515013
ABSTRACT
The chromatographic isolation and characterisation of the four compounds present in the quaternary phenanthridine veterinary trypanocidal agent, isometamidium chloride hydrochloride (ISM), is reported. The isolated compounds were unambiguously characterised using spectroscopic (NMR, UV, IR and MS) methods as 3-amino-8-[3-(3-carbamimidoyl-phenyl)-triazenyl]-5-ethyl-6-phenylethidium (1a) and related isomers, 8-amino-3-[3-(3-carbamimidoyl-phenyl)-triazenyl]-5-ethyl-6-phenylethidium, 3,-8-diamino-7-[3-(3-carbamimidoyl-phenyl)-triazenyl]-5-ethyl-6-phenylethidium and 3,-8-bis[3-(3-carbamimidoyl-phenyl)-triazenyl]-5-ethyl-6-phenylethidium. During the course of this study, it was realised that the nature of the solvent used in the NMR study was critical as in DMSO-d6 the quaternary group in the compounds was reduced to dihydro forms (e.g. 2a).
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Base de dados:
MEDLINE
Assunto principal:
Fenantridinas
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Espectrofotometria Ultravioleta
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Espectrometria de Massas
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Tripanossomicidas
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Espectroscopia de Ressonância Magnética
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Cromatografia Líquida de Alta Pressão
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Compostos de Amônio Quaternário
Idioma:
En
Ano de publicação:
2015
Tipo de documento:
Article