Instant live-cell super-resolution imaging of cellular structures by nanoinjection of fluorescent probes.
Nano Lett
; 15(2): 1374-81, 2015 Feb 11.
Article
em En
| MEDLINE
| ID: mdl-25533766
ABSTRACT
Labeling internal structures within living cells with standard fluorescent probes is a challenging problem. Here, we introduce a novel intracellular staining method that enables us to carefully control the labeling process and provides instant access to the inner structures of living cells. Using a hollow glass capillary with a diameter of <100 nm, we deliver functionalized fluorescent probes directly into the cells by (di)electrophoretic forces. The label density can be adjusted and traced directly during the staining process by fluorescence microscopy. We demonstrate the potential of this technique by delivering and imaging a range of commercially available cell-permeable and nonpermeable fluorescent probes to cells.
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Base de dados:
MEDLINE
Assunto principal:
Osteossarcoma
/
Corantes Fluorescentes
Limite:
Humans
Idioma:
En
Ano de publicação:
2015
Tipo de documento:
Article