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Long-term in vitro cultivation of Borrelia miyamotoi.
Margos, Gabriele; Stockmeier, Sylvia; Hizo-Teufel, Cecilia; Hepner, Sabrina; Fish, Durland; Dautel, Hans; Sing, Andreas; Dzaferovic, Eldina; Rieger, Melissa; Jungnick, Sabrina; Binder, Katrin; Straubinger, Reinhard K; Fingerle, Volker.
Afiliação
  • Margos G; Bavarian Health and Food Safety Authority, Oberschleissheim, Germany; German National Reference Centre for Borrelia, Oberschleissheim, Germany. Electronic address: gabriele.margos@lgl.bayern.de.
  • Stockmeier S; Bavarian Health and Food Safety Authority, Oberschleissheim, Germany; German National Reference Centre for Borrelia, Oberschleissheim, Germany.
  • Hizo-Teufel C; Bavarian Health and Food Safety Authority, Oberschleissheim, Germany; German National Reference Centre for Borrelia, Oberschleissheim, Germany.
  • Hepner S; German National Reference Centre for Borrelia, Oberschleissheim, Germany.
  • Fish D; Yale School of Public Health, New Haven, CT, USA.
  • Dautel H; IS Insect Services GmbH, Berlin, Germany.
  • Sing A; Bavarian Health and Food Safety Authority, Oberschleissheim, Germany.
  • Dzaferovic E; German National Reference Centre for Borrelia, Oberschleissheim, Germany.
  • Rieger M; German National Reference Centre for Borrelia, Oberschleissheim, Germany; Ludwig-Maximilians-University, Munich, Germany.
  • Jungnick S; German National Reference Centre for Borrelia, Oberschleissheim, Germany; Ludwig-Maximilians-University, Munich, Germany.
  • Binder K; German National Reference Centre for Borrelia, Oberschleissheim, Germany.
  • Straubinger RK; Ludwig-Maximilians-University, Munich, Germany.
  • Fingerle V; Bavarian Health and Food Safety Authority, Oberschleissheim, Germany; German National Reference Centre for Borrelia, Oberschleissheim, Germany.
Ticks Tick Borne Dis ; 6(2): 181-4, 2015 Mar.
Article em En | MEDLINE | ID: mdl-25561082
ABSTRACT
Borrelia are fastidious bacteria some of which are difficult to grow in vitro. Here, we report a method for successful continuous in vitro cultivation of the emerging pathogen Borrelia miyamotoi. The type and quantity of serum as well as the atmosphere were critical for successful in vitro cultivation. Optimal growth was achieved using 50% pooled human serum and an atmosphere of 6% CO2.
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Febre Recorrente / Borrelia / Ixodes / Meios de Cultura Limite: Animals / Humans Idioma: En Ano de publicação: 2015 Tipo de documento: Article
Texto completo
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Febre Recorrente / Borrelia / Ixodes / Meios de Cultura Limite: Animals / Humans Idioma: En Ano de publicação: 2015 Tipo de documento: Article