Fluorescent silver nanocluster DNA probes for multiplexed detection using microfluidic capillary electrophoresis.
Analyst
; 140(5): 1609-15, 2015 Mar 07.
Article
em En
| MEDLINE
| ID: mdl-25601044
ABSTRACT
DNA-stabilized fluorescent silver nanoclusters (AgNC DNA) are a new class of fluorophore that are formed by sequence specific interactions between silver and single-stranded DNA. By incorporating both target-binding and fluorescent-reporting sequences into a single synthetic DNA oligomer, AgNC DNA probes eliminate the need to conjugate dye or quencher molecules. In this study, we modify a AgNC DNA probe to demonstrate single-color multiplexed detection of DNA targets. We show that appending different lengths of poly-dT to the probe sequences tunes the electrophoretic mobility of AgNC DNA probes without affecting their fluorescence spectra. We use this to introduce a set of AgNC DNA probes selective for Hepatitis A, B and C target sequences that can be processed together in a simple, single-step protocol and distinguished with a resolution of 3.47 and signal to noise ratio of 17.23 in under 10 seconds by microfluidic capillary electrophoresis.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Prata
/
DNA Viral
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Sondas de DNA
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Eletroforese Capilar
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Microfluídica
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Nanoestruturas
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Nanopartículas Metálicas
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Corantes Fluorescentes
Tipo de estudo:
Diagnostic_studies
Limite:
Humans
Idioma:
En
Ano de publicação:
2015
Tipo de documento:
Article