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Self-assembled supramolecular nanoprobes for ratiometric fluorescence measurement of intracellular pH values.
He, Leiliang; Yang, Xiaohai; Zhao, Fang; Wang, Kemin; Wang, Qing; Liu, Jianbo; Huang, Jin; Li, Wenshan; Yang, Meng.
Afiliação
  • He L; State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Key Laboratory for Bio-Nanotechnology and Molecular Engineering of Hunan Province, Hunan University , Changsha 410082, People's Republic of China.
Anal Chem ; 87(4): 2459-65, 2015 Feb 17.
Article em En | MEDLINE | ID: mdl-25610947
Self-assembly of small building blocks into functional supramolecular nanostructure has opened prospects for the design of novel materials. With this molecular engineering strategy, we have developed self-assembled supramolecular nanoprobes (SSNPs) for ratiometric fluorescence measurement of pH values in cells. The nanoprobes with a diameter of ∼30 nm could be formulated just by mixing pH-sensitive adamantane-fluorescein (Ad-F) and pH-insensitive adamantane-Rhodamine B (Ad-R) with ß-cyclodextrin polymer (poly-ß-CD) at one time. The nanoprobes with good biocompatibility have been successfully applied to measure intracellular pH in the pH range of 4-8 and estimate pH fluctuations associated with different stimuli in cells. Moreover, we expect that this self-assembled approach is applicable to the construction of nanoprobes for other targets in cells just by replacing the respective indicator dyes with relevant indicators.
Assuntos

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Substâncias Macromoleculares / Nanopartículas / Corantes Fluorescentes / Medições Luminescentes Limite: Humans Idioma: En Ano de publicação: 2015 Tipo de documento: Article

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Substâncias Macromoleculares / Nanopartículas / Corantes Fluorescentes / Medições Luminescentes Limite: Humans Idioma: En Ano de publicação: 2015 Tipo de documento: Article