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Insight into the bronchodilator activity of Vitex negundo.
Khan, Munasib; Shah, Abdul Jabbar; Gilani, Anwarul Hassan.
Afiliação
  • Khan M; Natural Product Research Unit, Department of Biological and Biomedical Sciences, Aga Khan University Medical College , Karachi , Pakistan .
Pharm Biol ; 53(3): 340-4, 2015 Mar.
Article em En | MEDLINE | ID: mdl-25622948
ABSTRACT
CONTEXT Vitex negundo Linn. (Verbenaceae) is traditionally used in hyperactive respiratory disorders.

OBJECTIVE:

This study explored the mechanisms underlying the effectiveness of Vitex negundo in hyperactive respiratory disorders. MATERIALS AND

METHODS:

Crude extract of V. negundo leaves was obtained. For in vivo bronchodilatory activity in anesthetized rats, different doses (1, 3, 10, 30, and 50 mg/kg) of the crude extract of V. negundo (Vn.Cr) were tested. The underlying mechanisms were studied in isolated guinea pig tracheal strips, suspended in organ baths at 37 °C.

RESULTS:

Intravenous doses of the crude extract of Vn.Cr showed dose-dependent bronchodilatory effect (9-50%) against carbachol (CCh; 100 µg/kg)-induced bronchoconstriction, similar to aminophylline. In isolated guinea-pig tracheal strips, Vn.Crrelaxed CCh (1 µM) and high K(+) pre-contractions with respective EC50 values of 0.72 (0.48-1.10; n = 5) and 3.38 mg/mL (1.84-6.21; n = 4), similar to papaverine. Diltiazem also relaxed both contractions with more potency against high K(+) pre-contraction (p < 0.05). Pre-incubation of the tracheal strips with Vn.Cr potentiated the isoprenaline inhibitory concentration response curves (CRCs), similar to papaverine.

DISCUSSION:

The inhibitory effect against CCh and high K(+) suggests involvement of phosphodiesterase (PDE) inhibitory pathway(s), in addition to an inhibitory effect on Ca(++) entry. This finding was further strengthened when pre-treatment of the tracheal strips potentiated the isoprenaline CRCs.

CONCLUSION:

RESULTS suggest Vn.Cr possesses a combination of papaverine-like PDE inhibitor and diltiazem-like Ca(++) entry blocking constituents, which partly explain its bronchodilatory effect, thus validating its medicinal importance in asthma.
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Traqueia / Broncodilatadores / Extratos Vegetais / Broncoconstrição / Vitex Limite: Animals Idioma: En Ano de publicação: 2015 Tipo de documento: Article

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Traqueia / Broncodilatadores / Extratos Vegetais / Broncoconstrição / Vitex Limite: Animals Idioma: En Ano de publicação: 2015 Tipo de documento: Article