Insight into the bronchodilator activity of Vitex negundo.
Pharm Biol
; 53(3): 340-4, 2015 Mar.
Article
em En
| MEDLINE
| ID: mdl-25622948
ABSTRACT
CONTEXT Vitex negundo Linn. (Verbenaceae) is traditionally used in hyperactive respiratory disorders. OBJECTIVE:
This study explored the mechanisms underlying the effectiveness of Vitex negundo in hyperactive respiratory disorders. MATERIALS ANDMETHODS:
Crude extract of V. negundo leaves was obtained. For in vivo bronchodilatory activity in anesthetized rats, different doses (1, 3, 10, 30, and 50 mg/kg) of the crude extract of V. negundo (Vn.Cr) were tested. The underlying mechanisms were studied in isolated guinea pig tracheal strips, suspended in organ baths at 37 °C.RESULTS:
Intravenous doses of the crude extract of Vn.Cr showed dose-dependent bronchodilatory effect (9-50%) against carbachol (CCh; 100 µg/kg)-induced bronchoconstriction, similar to aminophylline. In isolated guinea-pig tracheal strips, Vn.Crrelaxed CCh (1 µM) and high K(+) pre-contractions with respective EC50 values of 0.72 (0.48-1.10; n = 5) and 3.38 mg/mL (1.84-6.21; n = 4), similar to papaverine. Diltiazem also relaxed both contractions with more potency against high K(+) pre-contraction (p < 0.05). Pre-incubation of the tracheal strips with Vn.Cr potentiated the isoprenaline inhibitory concentration response curves (CRCs), similar to papaverine.DISCUSSION:
The inhibitory effect against CCh and high K(+) suggests involvement of phosphodiesterase (PDE) inhibitory pathway(s), in addition to an inhibitory effect on Ca(++) entry. This finding was further strengthened when pre-treatment of the tracheal strips potentiated the isoprenaline CRCs.CONCLUSION:
RESULTS suggest Vn.Cr possesses a combination of papaverine-like PDE inhibitor and diltiazem-like Ca(++) entry blocking constituents, which partly explain its bronchodilatory effect, thus validating its medicinal importance in asthma.Palavras-chave
Texto completo:
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Base de dados:
MEDLINE
Assunto principal:
Traqueia
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Broncodilatadores
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Extratos Vegetais
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Broncoconstrição
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Vitex
Limite:
Animals
Idioma:
En
Ano de publicação:
2015
Tipo de documento:
Article