Smart nanoprobes for the detection of alkaline phosphatase activity during osteoblast differentiation.
Chem Commun (Camb)
; 51(15): 3270-2, 2015 Feb 21.
Article
em En
| MEDLINE
| ID: mdl-25623488
Gold nanoparticle-conjugated fluorescent hydroxyapatite (AuFHAp) was developed as a smart nanoprobe for measuring alkaline phosphatase (ALP) activity. AuFHAp showed NIR fluorescence due to the hydrolysis of its phosphate groups by ALP. In addition, gold nanoparticles help reduce the nonspecific signal by absorbing nonspecific fluorescence. Through in vitro tests, we confirmed that the AuFHAp probe was capable of detecting ALP levels related to osteoblast activity in living cells with high fluorescence intensity.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Osteoblastos
/
Durapatita
/
Fosfatase Alcalina
/
Nanopartículas Metálicas
/
Ouro
Tipo de estudo:
Diagnostic_studies
Limite:
Animals
Idioma:
En
Ano de publicação:
2015
Tipo de documento:
Article