Two-subunit enzymes involved in eukaryotic post-transcriptional tRNA modification.
RNA Biol
; 11(12): 1608-18, 2014.
Article
em En
| MEDLINE
| ID: mdl-25625329
ABSTRACT
tRNA modifications are crucial for efficient and accurate protein translation, with defects often linked to disease. There are 7 cytoplasmic tRNA modifications in the yeast Saccharomyces cerevisiae that are formed by an enzyme consisting of a catalytic subunit and an auxiliary protein, 5 of which require only a single subunit in bacteria, and 2 of which are not found in bacteria. These enzymes include the deaminase Tad2-Tad3, and the methyltransferases Trm6-Trm61, Trm8-Trm82, Trm7-Trm732, and Trm7-Trm734, Trm9-Trm112, and Trm11-Trm112. We describe the occurrence and biological role of each modification, evidence for a required partner protein in S. cerevisiae and other eukaryotes, evidence for a single subunit in bacteria, and evidence for the role of the non-catalytic binding partner. Although it is unclear why these eukaryotic enzymes require partner proteins, studies of some 2-subunit modification enzymes suggest that the partner proteins help expand substrate range or allow integration of cellular activities.
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Base de dados:
MEDLINE
Assunto principal:
TRNA Metiltransferases
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RNA de Transferência
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Processamento Pós-Transcricional do RNA
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Subunidades Proteicas
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Proteínas de Saccharomyces cerevisiae
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Nucleosídeo Desaminases
Idioma:
En
Ano de publicação:
2014
Tipo de documento:
Article