Detection of benzo[a]pyrene-guanine adducts in single-stranded DNA using the α-hemolysin nanopore.
Nanotechnology
; 26(7): 074002, 2015 Feb 20.
Article
em En
| MEDLINE
| ID: mdl-25629967
ABSTRACT
The carcinogenic precursor benzo[a]pyrene (BP), a polycyclic aromatic hydrocarbon, is released into the environment through the incomplete combustion of hydrocarbons. Metabolism of BP in the human body yields a potent alkylating agent (benzo[a]pyrene diol epoxide, BPDE) that reacts with guanine (G) in DNA to form an adduct implicated in cancer initiation. We report that the α-hemolysin (αHL) nanopore platform can be used to detect a BPDE adduct to G in synthetic oligodeoxynucleotides. Translocation of a 41-mer poly-2'-deoxycytidine strand with a centrally located BPDE adduct to G through αHL in 1 M KCl produces a unique multi-level current signature allowing the adduct to be detected. This readily distinguishable current modulation was observed when the BPDE-adducted DNA strand translocated from either the 5' or 3' directions. This study suggests that BPDE adducts and other large aromatic biomarkers can be detected with αHL, presenting opportunities for the monitoring, quantification, and sequencing of mutagenic compounds from cellular DNA samples.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Benzo(a)pireno
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7,8-Di-Hidro-7,8-Di-Hidroxibenzo(a)pireno 9,10-óxido
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DNA de Cadeia Simples
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Adutos de DNA
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Nanoporos
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Guanina
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Proteínas Hemolisinas
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Neoplasias
Tipo de estudo:
Diagnostic_studies
Limite:
Humans
Idioma:
En
Ano de publicação:
2015
Tipo de documento:
Article