In vivo visualization and quantification of mitochondrial morphology in C. elegans.
Methods Mol Biol
; 1265: 367-77, 2015.
Article
em En
| MEDLINE
| ID: mdl-25634288
ABSTRACT
Caenorhabditis elegans is a highly malleable model system, intensively used for functional, genetic, cytometric, and integrative studies. Due to its simplicity and large muscle cell number, C. elegans has frequently been used to study mitochondrial deficiencies caused by disease or drug toxicity. Here, we describe a robust and efficient method to visualize and quantify mitochondrial morphology in vivo. This method has many practical and technical advantages above traditional (manual) methods and provides a comprehensive analysis of mitochondrial morphology.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Caenorhabditis elegans
/
Microscopia Confocal
/
Microscopia de Fluorescência
/
Mitocôndrias
Tipo de estudo:
Guideline
Limite:
Animals
Idioma:
En
Ano de publicação:
2015
Tipo de documento:
Article