Identification of essential genes and synthetic lethal gene combinations in Escherichia coli K-12.
Methods Mol Biol
; 1279: 45-65, 2015.
Article
em En
| MEDLINE
| ID: mdl-25636612
ABSTRACT
Here we describe the systematic identification of single genes and gene pairs, whose knockout causes lethality in Escherichia coli K-12. During construction of precise single-gene knockout library of E. coli K-12, we identified 328 essential gene candidates for growth in complex (LB) medium. Upon establishment of the Keio single-gene deletion library, we undertook the development of the ASKA single-gene deletion library carrying a different antibiotic resistance. In addition, we developed tools for identification of synthetic lethal gene combinations by systematic construction of double-gene knockout mutants. We introduce these methods herein.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Genes Essenciais
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Genômica
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Escherichia coli K12
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Genes Bacterianos
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Genes Letais
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Genes Sintéticos
Tipo de estudo:
Diagnostic_studies
/
Prognostic_studies
Idioma:
En
Ano de publicação:
2015
Tipo de documento:
Article