Enzymatic preparation of multimilligram amounts of pure single-stranded DNA samples for material and analytical sciences.
Anal Biochem
; 475: 68-73, 2015 Apr 15.
Article
em En
| MEDLINE
| ID: mdl-25637680
ABSTRACT
We present a method for high-yield production of multimilligram amounts of pure single-stranded DNA employing rolling circle amplification (RCA) and processing by restriction enzymes. Pure and homogeneous samples are produced with minimal handling time, reagents, and waste products. The RCA method is more than twice as efficient in dNTP incorporation than conventional polymerase chain reaction in producing end product. The validity and utility of the method are demonstrated in the production of a uniformly (13)C/(15)N-labeled 38-nt cocaine aptamer DNA used in nanosensing devices.
Palavras-chave
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
DNA de Cadeia Simples
/
Aptâmeros de Nucleotídeos
Idioma:
En
Ano de publicação:
2015
Tipo de documento:
Article