Perlecan is recruited by dystroglycan to nodes of Ranvier and binds the clustering molecule gliomedin.
J Cell Biol
; 208(3): 313-29, 2015 Feb 02.
Article
em En
| MEDLINE
| ID: mdl-25646087
ABSTRACT
Fast neural conduction requires accumulation of Na(+) channels at nodes of Ranvier. Dedicated adhesion molecules on myelinating cells and axons govern node organization. Among those, specific laminins and dystroglycan complexes contribute to Na(+) channel clustering at peripheral nodes by unknown mechanisms. We show that in addition to facing the basal lamina, dystroglycan is found near the nodal matrix around axons, binds matrix components, and participates in initial events of nodogenesis. We identify the dystroglycan-ligand perlecan as a novel nodal component and show that dystroglycan is required for the selective accumulation of perlecan at nodes. Perlecan binds the clustering molecule gliomedin and enhances clustering of node of Ranvier components. These data show that proteoglycans have specific roles in peripheral nodes and indicate that peripheral and central axons use similar strategies but different molecules to form nodes of Ranvier. Further, our data indicate that dystroglycan binds free matrix that is not organized in a basal lamina.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Nós Neurofibrosos
/
Moléculas de Adesão Celular Neuronais
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Proteoglicanas de Heparan Sulfato
Limite:
Animals
/
Humans
Idioma:
En
Ano de publicação:
2015
Tipo de documento:
Article