Emergence of ß-lactamases and extended-spectrum ß-lactamases (ESBLs) producing Salmonella in retail raw chicken in China.
Foodborne Pathog Dis
; 12(3): 228-34, 2015 Mar.
Article
em En
| MEDLINE
| ID: mdl-25658910
ABSTRACT
ß-Lactamases and extended-spectrum ß-lactamases (ESBLs) producing pathogenic bacteria were widely studied previously in China, but were seldom focused on foodborne Salmonella. In this study, an investigation concerning ß-lactamases and ESBLs producing Salmonella recovered from retail raw chickens was performed. Sixty of 699 foodborne Salmonella isolates were detected as ß-lactamases and ESBLs-producing ones that covered 12 Salmonella serotypes and exhibited different pulsed-field gel electrophoresis genotypes. Forty-four of 60 ß-lactamases and ESBLs-producing strains were simultaneously resistant to ampicillin, amoxicillin/clavulanic acid, ceftiofur, ceftriaxone, and cefoxitin. The most commonly detected ß-lactamases and ESBLs-encoding gene was bla(TEM-1) (n = 44), followed by bla(OXA-1) (n = 38), bla(CMY-2) (n = 29), bla(PSE-1-like) (n = 1), bla(CTX-M-3) (n = 16), and bla(CTX-M-15) (n = 1), respectively. Fourteen, 24, 21, and 1 isolates were detected simultaneously positive for 1, 2, 3, and 4 of the detected ß-lactamases and ESBLs-encoding genes, respectively. A Salmonella strain simultaneously co-carrying bla(TEM-1), bla(OXA-1), bla(CMY-2), and bla(CTX-M-3) was first reported in the present study. Amino acid substitution of Trp244Cys/His247Leu was detected in PSE-1, Val218Asp in CMY-2, and Asp242Gly in CTX-M-15 enzymes, respectively. A difference was found among the amino acid sequences of the detected OXA-1, CMY-2, CTX-M, PSE-1, and TEM-1. The results demonstrated that ß-lactamases and ESBLs were emerging and prevalent in foodborne Salmonella.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Salmonella
/
Beta-Lactamases
/
Farmacorresistência Bacteriana Múltipla
/
Carne
/
Antibacterianos
Limite:
Animals
País como assunto:
Asia
Idioma:
En
Ano de publicação:
2015
Tipo de documento:
Article