Rapid evaporative ionization mass spectrometry imaging platform for direct mapping from bulk tissue and bacterial growth media.
Anal Chem
; 87(5): 2527-34, 2015 Mar 03.
Article
em En
| MEDLINE
| ID: mdl-25671656
ABSTRACT
Rapid evaporative ionization mass spectrometry (REIMS) technology allows real time intraoperative tissue classification and the characterization and identification of microorganisms. In order to create spectral libraries for training the classification models, reference data need to be acquired in large quantities as classification accuracy generally improves as a function of number of training samples. In this study, we present an automated high-throughput method for collecting REIMS data from heterogeneous organic tissue. The underlying instrumentation consists of a 2D stage with an additional high-precision z-axis actuator that is equipped with an electrosurgical diathermy-based sampling probe. The approach was validated using samples of human liver with metastases and bacterial strains, cultured on solid medium, belonging to the species P. aeruginosa, B. subtilis, and S. aureus. For both sample types, spatially resolved spectral information was obtained that resulted in clearly distinguishable multivariate clustering between the healthy/cancerous liver tissues and between the bacterial species.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Bactérias
/
Diagnóstico por Imagem
/
Neoplasias Colorretais
/
Adenocarcinoma
/
Meios de Cultura
/
Espectrometria de Massas por Ionização por Electrospray
/
Neoplasias Hepáticas
Tipo de estudo:
Diagnostic_studies
Limite:
Humans
Idioma:
En
Ano de publicação:
2015
Tipo de documento:
Article