Aminobenzofuran-fused rhodamine dyes with deep-red to near-infrared emission for biological applications.
J Org Chem
; 80(6): 3170-5, 2015 Mar 20.
Article
em En
| MEDLINE
| ID: mdl-25692322
ABSTRACT
Aminobenzofuran-fused rhodamine dyes (AFR dyes) containing an amino group were constructed by an efficient condensation based on 3-coumaranone derivatives. AFR dyes exhibited significantly improved properties, including deep-red and near-infrared emissions, a large Stokes shift, good photostability, and wide pH stability. 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazolium assay experiments show that these AFR dyes are biocompatible for their low cytotoxicity to both A549 and HeLa cells. Cell imaging data reveal that AFR1, AFR1E, and AFR2 are mainly located in the mitochondria, while AFR3 is a lysosome tracker. As far as we know, NIR AFR3 is the longest fluorescent rhodamine derivative containing the amino group. These amino group-containing AFR dyes hold great potential in fluorogenic detection, biomolecule labeling, and cell imaging.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Rodaminas
/
Benzofuranos
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Materiais Biocompatíveis
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Corantes Fluorescentes
Limite:
Humans
Idioma:
En
Ano de publicação:
2015
Tipo de documento:
Article